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Tel:031-315-6644, 010-3721-2201     Fax:0303-3441-3345

























Spin and Vacuum column type

Purified ds or ssDNA in 5 minutes from PCR amplification

Up to 95% recovery of DNA fragments (70 bp-10 kb)

> 99.5% primer removal

No removal of mineral oil necessary

Economical Kits

Excellent reproducibility

Total procedure is 5 min

























Nucleogen PCR Purification Kit is designed for fast cleanup of DNA fragments from PCR or other enzymatic reactions. Using silica gel membrane technology, Nucleogen PCR Purification Kit deliver high-purity DNA suitable for use in all applications.































50 preps  … 5315

200 preps  … 5312


























Nucleogen PCR Purification Kits contain a silica gel membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.


The Nucleogen PCR Purification system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the PCR sample or other enzymatic reaction, and the mixture is applied to the Nucleogen PCR Purification spin column. Nucleic acids adsorb to the silica gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.


DNA fragments purified with the Nucleogen PCR Purification Kits are ready for direct use in most applications, including:

Automated fluorescent sequencing, including capillary sequencing
Radioactive sequencing
Microarray analysis
Ligation and transformation
Restriction digestion
In vitro transcription

























       a    b    M    a    b




- 1100 bp

- 94 bp

Analysis of PCR products before (a) and after (b) purification with the Nucleogen PCR Purification Kit(1312).
Sample were analyzed on a 1% TAE agarose gel.



























Kit Contents

Spin Columns, Collection Tubes, PCR Purification Buffer, Wash A' solution, Elution Buffer